Light scatter is independent of fluorescence.
Visible light scatter is measured in two different directions, the forward direction (Forward Scatter or FSC) which can indicate the relative size of the cell and at 90° (Side Scatter or SSC) which indicates the internal complexity or granularity of the cell. Each particle is analyzed for visible light scatter and one or multiple fluorescence parameters. It has seen dramatic advances over the last 30 years, allowing unprecedented detail in studies of the immune system and other areas of cell biology.įlow cytometry is a technology that rapidly analyzes single cells or particles as they flow past single or multiple lasers while suspended in a buffered salt-based solution. These include, fluorescently conjugated antibodies, DNA binding dyes, viability dyes, ion indicator dyes and fluorescent expression proteins.įlow cytometry is a powerful tool that has applications in immunology, molecular biology, bacteriology, virology, cancer biology and infectious disease monitoring. Cell populations can be analyzed and/or purified based on their fluorescent or light scattering characteristics.Ī variety of fluorescent reagents are utilized in flow cytometry. These signals are converted into electronic signals that are analyzed by a computer and written to a standardized format (.fcs) data file. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals that are read by detectors such as photodiodes or photomultiplier tubes.
Flow cytometry is a technology that provides rapid multi-parametric analysis of single cells in solution.
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